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Abstract
A rapid and precise colorimetric method for estimation of 5- fluorouracil in bulk drug and dosage forms.
Author(s): SK Banerjee, A Sumathi
Pharmacy Dept., Faculty of Technology and Engineering, M.S. University of Baroda, Kalabhavan, Baroda 390001
Correspondence Address:
S K Banerjee Pharmacy Dept., Faculty of Technology and Engineering, M.S. University of Baroda, Kalabhavan, Baroda 390001
Pharmacy Dept., Faculty of Technology and Engineering, M.S. University of Baroda, Kalabhavan, Baroda 390001
Correspondence Address:
S K Banerjee Pharmacy Dept., Faculty of Technology and Engineering, M.S. University of Baroda, Kalabhavan, Baroda 390001
The proposed colorimetric method for estimation of 5-Fluorouracil (5-FU) is based on the reaction between the drug and copper acetate in 3:2 chloroform : methanol mixture in presence of 0.5 percent diethylamine (DEA). The yellowish green coloured complex shows a lamda max at 350 nm and calibration curve was obtained within the range 0.4-11.0 mug/ml. The method is statistically validated and results compared favourably with those obtained by the reported method.
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