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Abstract
A New Reverse Phase High Performance Liquid Chromatographic Method For Analysis Of Rofecoxib In Tablets
Author(s): K. E. V Nagoji, S Vijayasrinivas, M Kiran Kumar, N Mathivanan, M Satish Kumar, M. E. B Rao
A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the determination of rofecoxib in tablets. The analyte is resolved by using a mobile phase (methanol and water in the ratio 50:50) at a flow rate of 1 ml/min on an isocratic HPLC system (Shimadzu) consisting of LC 10AT liquid pump, SPD 10A UV-Visible detector, a ODS C-18 RP column (4.6 mm I.DX25 cm) at a wavelength of 230 nm. The linear dynamic range for rofecoxib was 2-40 μg/ml by this method. Paracetamol was used as an internal standard.
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