Abstract
A Global Gene Expression Study on Xiao Xian Xiong Decoction-Treated Hone-1
M Kandiah Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Kampar, Perak 31900, 1Faculty of Dentistry, University of Malaya, Kuala Lumpur 50603, Malaysia, 2Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong 999077, China
Correspondence Address:
Yang Mooi Lim, M Kandiah Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Kampar, Perak 31900, Malaysia, E-mail: ymlim@utar.edu.my
In Malaysia, nasopharyngeal carcinoma is the 5th (4.0 %) most prevalent malignant tumor and it is the 3rd highest in male for cancer occurrence rate (8.4 %). Therefore, discovery of novel anti-cancer herbal drugs is of importance. In this study, the cytotoxic effect of traditional Chinese herbal prescription Xiao Xian Xiong decoction which made up of Huanglian (Coptis chinensis Franch.), Banxia (Pinellia ternata (Thunb.) Breit.), and Gualou (Trichosanthes kirilowaii Maxim) was tested. The cytotoxic effect Xiao Xian Xiong decoction was studied on 8 nasopharyngeal cancer cell lines (TWO-1, TWO-4, HONE-1, SUNE-1, CNE-2, HK-1, CNE-1 and C666-10). Global gene expression analysis was conducted using nCounter XT gene expression assay. The half-maximal inhibitory concentration values obtained for Xiao Xian Xiong decoction-treated HONE-1 and CNE-2 cell lines were 88.55 and 92.42 μg/ml respectively. Global gene expression showed Xiao Xian Xiong decoction significantly downregulated genes that are associated with cell growth and proliferation, whereas upregulated genes that are associated with induced cell cycle arrest and apoptosis in HONE-1 cells.
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