Abstract

The condition known as benign prostatic hyperplasia, which causes enlargement in the prostate gland and male pattern hair loss are both treated with a family of drugs known as 5 alpha reductase inhibitors. This study shows that Solanum nigrum L. has 5 alpha reductase inhibitory action, that is helpful in the treatment of androgenic diseases. It was carried out to screen for phytochemicals and to analyze high-performance thin layer chromatography. Using petroleum ether and methanol extract, the 5 alpha reductase inhibitory activity of the plant was compared to the renowned 5 alpha-reductase inhibitor finasteride. The inhibition of extracts to the enzyme was assessed using a biochemical approach to measure the activity of 5 alpha-reductase. Because the substrate nicotinamide adenine dinucleotide phosphate has a particular absorbance at 340 nm, the optical density value of each sample was monitored with an ultraviolet spectrophotometer. Nicotinamide adenine dinucleotide phosphate concentration rises over time in the presence of a 5 alpha-reductase inhibitor because the enzyme 5 alpha-reductase needs nicotinamide adenine dinucleotide phosphate as a substrate. Thus, this technique implies 5 alpha-reductase activity. The method was quite successful in evaluating the plant capacity to block 5 alpha-reductase. It was determined that Solanum nigrum L. petroleum ether extract 125.528±0.634 (μg/ml) and linoleic acid (chemical biomarker of the plant material) 89.672±0.669 (μg/ml) were promising candidates for future investigation into their anti-androgenic activities.